Microfluidics technology for sex-sorting of bovine sperms
Principal Investigator
Prof. Ashis Kumar Sen
Objective
- To measure the physical properties of X and Y-bearing bovine sperm cells., To design a microfluidic device for sorting X and Y-bearing bovine sperms., The demonstrate sorting of bovine sperms and characterize purity and throughput., To evaluate biological characteristics (DNA integrity, etc.) of sorted sperms., To develop an industrial prototype and demonstrate sperm sorting.
Description
- Sexed semen generation and artificial insemination are two important steps toward the larger objective of boosting milk production. Sperm sexing has the potential to significantly improve the quality and quantity of production in the livestock industries., Presently, the only effective sperm-sorting system is flow cytometry, a high-speed analysis tool for the sorting of cells. The flow cytometry sex-sorting method is detrimental to the sperm cells, especially affecting their viability., Microfluidic-based sorting appears to be more suitable. Microfluidics enables sperm cells to be sorted in a simpler, time-effective, and gentler manner that more closely resembles natural selection processes while avoiding harmful aspects of conventional sperm-sorting techniques.
Impact
- New technology and devices for sexed sorting of bovine sperm cells., IP and Licensing.
Budget in Lakhs
50.00
Duration
3 Years