Equipment Support: Identification of biomarkers and novel drug targets for breast cancer from Indian cohorts using next generation sequencing
Principal Investigator
Prof. S. Mahalingam
Objective
- The main aim of this proposal is to identify the pediatric leukemia specific biomarker gene panel for early detection, understand the treatment responses, disease progression and novel drug targets for Indian cohorts.
Description
- Pediatric leukemia remains a significant contributor to childhood lethality rates. However, recent development of new technologies including next-generation sequencing (NGS) has increased our understanding of the biological and genetic underpinnings of leukemia, resulting in reasonable diagnostic and treatment paradigms. To the best of our knowledge, there is no genomic information is available for Indian pediatric leukemia. In the present proposal we plan to examine the longitudinal restructuring of the whole exome and transcriptome over five years and evaluates whether change in gene mutation and expression profile as biomarker for pediatric leukemia in India.
Impact
- We need to identify and isolate cancer cells from the total blood cells of pediatric leukemia patient samples, which is critical to carry out the cancer cell specific genomic sequencing. Furthermore, flow cytometer is required to identify the stages and grade of the leukiemia using fluorescent tagged antibodies.
Budget in Lakhs
92.00